Dipeptidyl Peptidase IV (DPP4) Inhibitor Screening Assay Kit (E-BC-D007)
For research use only.
| Detection Principle | Dipeptide base peptidase 4 (Dipeptidyl peptidase 4, DPP4), also known as CD26, is a kind of serine protease, can decompose the peptide chain N the second place, proline and alanine residue peptide bond. DPP4 can quickly decompose enterotropin in living organisms, which can stabilize insulin levels and promote a decrease in blood sugar levels in the body. The detection principle of this kit is that DPP4 can decompose the substrate and release fluorescent substance AMC. The addition of DPP4 inhibitor can inhibit the enzyme activity, and the inhibitory ability of the inhibitor can be determined by the fluorescence value. |
| Sample Type | Inhibitor |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=360 nm/460 nm) |
| Research Area | Inhibitor Screening |
| Other Reagents Required | Double distilled water |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Precision | inter-assay CV: 5.7-7.2% | intra-assay CV: 0.6-2.7% |
| Sample Volume | 30 μL |
| Assay Time | 1 h 10 min |
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