For research use only.
| Verified Samples |
Verified Samples in WB: 293T, HT29, A172 Verified Samples in IHC: Human liver cancer |
| Dilution | WB 1:500-1:2000, IHC 1:50-1:300 |
| Clonality | Polyclonal |
| Immunogen | Fusion protein of human EIF3K |
| Abbre | EIF3K |
| Synonyms | ARG134, EIF3-p28, EIF3K, EIF3S12, Eukaryotic translation initiation factor 3 subunit 12, Eukaryotic translation initiation factor 3 subunit K, HSPC029, M9, MSTP001, Muscle specific gene M9 protein, Muscle-specific gene , eIF 3 p25, eIF 3 p28, eIF-3 p25, eIF-3 p28, eif3k |
| Swissprot | |
| Calculated MW | 25 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus. Cytoplasm. |
| Concentration | 1.32 mg/mL |
| Buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
| Purification Method | Antigen affinity purification |
| Research Areas | Epigenetics and Nuclear Signaling, Neuroscience |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | The 700-kD eukaryotic translation initiation factor-3 (eIF3) is the largest eIF and contains at least 12 subunits, including EIF2S12. eIF3 plays an essential role in translation by binding directly to the 40S ribosomal subunit and promoting formation of the 40S preinitiation complex. |
Other Clones
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