Elab Fluor® 647-conjugated beta actin Monoclonal Antibody (AN00295M)
For research use only.
| Verified Samples |
Verified Samples in FCM: C6 cells were transfected with an expression plasmid to prepare an overexpression cell model |
| Dilution | FCM 0.2 μg per 106 cells in a 100 μL suspension |
| Isotype | IgG1 |
| Host | Mouse |
| Reactivity | Rat |
| Applications | FCM |
| Clonality | Monoclonal |
| Immunogen | Recombinant human Beta actin protein by E. Coli |
| Abbre | beta actin |
| Synonyms | A26C1A, ACTB, Actin beta, Actin cytoplasmic 1, Actx, b actin |
| Swissprot | |
| Cellular Localization | Cytoplasm>cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.05% Proclin300, 1% protective protein and 50% glycerol, pH7.4 |
| Purification Method | Protein A/G Purification |
| Research Areas | Signal Transduction, Tags and Cell Markers, Isotype/Loading, Controls, Neuroscience, Biochemicals, Cancer, Cardiovascular, Cell Biology, Immunology, Metabolism, Epigenetics and Nuclear Signaling, Stem Cells |
| Clone No. | 3A9 |
| Conjugation | Elab Fluor®647 |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
| Amax | 650nm |
| Emax | 665nm |
| background | Beta-actin (ACTB/ACTB) is one of six different actin isoforms in humans. Actins are highly conserved proteins that are involved in cell motility,structure and integrity. Beta actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. Beta-actin gene is stably and constitutively expressed at high levels in most tissues and cells,and commonly used as a loading control for western blot and as a control for qPCR. |
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Elab Fluor®647
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