epsilon Tubulin Monoclonal Antibody (E-AB-22138)
For research use only.
| Verified Samples |
Verified Samples in WB: MCF-7, Rat brain, Mouse brain |
| Dilution | WB 1:1000-2000 |
| Isotype | IgG |
| Host | Mouse |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal |
| Immunogen | Synthetic Peptide of Epsilon Tubulin |
| Abbre | epsilon Tubulin |
| Synonyms | Epsilon-tubulin, FLJ22589, FLJ44203, OTTHUMP00000040425, TBE, TUBE, TUBE 1, TUBE1, Tubulin epsilon, Tubulin epsilon 1, Tubulin epsilon chain, dJ142L7.2 |
| Swissprot | |
| Observed MW |
55 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm>cytoskeleton>centrosome. Associated with pericentriolar material. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
| Purification Method | Protein A purification |
| Research Areas | Signal Transduction |
| Clone No. | 2B4 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | This gene encodes a member of the tubulin superfamily. This protein localizes to the centriolar sub-distal appendages that are associated with the older of the two centrioles after centrosome duplication. This protein plays a central role in organization of the microtubules during centriole duplication. A pseudogene of this gene is found on chromosome 5. TUBE1 (Tubulin Epsilon 1) is a Protein Coding gene. Diseases associated with TUBE1 include Chronic Frontal Sinusitis and Frontal Sinusitis. Among its related pathways are Sertoli-Sertoli Cell Junction Dynamics and Chks in Checkpoint Regulation. GO annotations related to this gene include GTP binding and structural constituent of cytoskeleton. An important paralog of this gene is TUBB4B. |
Other Clones
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Other Formats
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Unconjugated
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