Toll-free:1-888-852-8623

All categories

  • All categories
  • Flow Cytometry Antibodies
  • ELISA Kits
  • MACS Cell Isolation
  • Antibodies and Reagents
  • Cell Health Detection
  • Metabolism Assays
  • Immunoassays
  • Cell Identification
  • Proteins and Peptides
  • Cell Culture
Please enter the item number/product keyword!
Keyword cannot be empty !
INSERT SYMBOLS:
  • α
  • β
  • γ
  • δ
  • ε
  • ζ
  • η
  • θ
  • κ
  • μ
  • ω
  • σ
  • τ
  • λ
  • ⅩⅢ
  • ⅩⅢ
  • ⅩⅣ
  • ⅩⅤ
  • ⅩⅦ
  • ⅩⅧ
  • UP ↑
All Size Price Qty
200μL $ 410.00
120μL $ 260.00
60μL $ 160.00
Add to cart

For research use only.

Verified Samples Verified Samples in WB: Hela, HepG2, NIH/3T3, Mouse lung, Mouse kidney, Mouse brain, Rat lung, Rat kidney, Rat brain
Dilution WB 1:500-1:2000
Isotype IgG
Host Mouse
Reactivity Human,  Mouse,  Rat
Applications WB
Clonality Monoclonal
Immunogen Recombinant protein corresponding to Mouse ERK2
Abbre ERK1/2
Synonyms ERK-1,  ERK1,  ERT2,  Extracellular signal-regulated kinase 1,  Insulin-stimulated MAP2 kinase,  MAP kinase 3,  MAP kinase isoform p44,  MAPK 3,  MAPK3,  Microtubule-associated protein 2 kinase,  Mitogen-activated protein kinase 3,  PRKM3,  p44-MAPK
Swissprot
Calculated MW 42/44 kDa
Observed MW 42 /44 kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Concentration 3.2 mg/mL
Buffer PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH7.4
Purification Method Affinity purification
Research Areas Cancer,  Neuroscience,  Signal Transduction,  Stem Cells
Clone No. 4H6A4
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
Cat.No. Product Name Sizes
E-AB-1001 Goat Anti-Mouse IgG(H+L)(peroxidase/HRP conjugated) 500μL , 120μL , 60μL
E-AB-1024 Goat Anti-Mouse IgG(H+L)(Biotin conjugated) 500μL , 120μL , 60μL
E-AB-1043 Streptavidin(peroxidase/HRP conjugated) 500μL , 120μL , 60μL
E-AB-1196 HRP-Goat Anti-Mouse IgG(H+L) preadsorbed 500μL , 120μL , 1mL
E-IR-R304A Western Blot Detection Kit 50Assays
E-IR-R304B High Accuracy and Absorbability Western Blot Detection Kit 50Assays
Other Clones

{{antibodyDetailsPage.numTotal}} Results

Other Formats

{{formatDetailsPage.numTotal}} Results

Unconjugated

  • Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}