Fatty Acid Uptake Fluorometric Assay Kit (E-BC-F067)

For research use only.
Detection Principle |
Fatty acid is an important source of energy for the body. Fatty acid intake capacity has been associated with diseases such as obesity and diabetes, and is therefore an important target for the treatment of diseases such as obesity, Ⅱ diabetes, and fatty liver. Hyperactive cancer cells have a strong fatty acid uptake capacity, so the fatty acid uptake capacity is also used as an important indicator of cancer drug development, and the fatty acid pathway is also attracted attention as one of the targets of cancer drug development. This kit provides a convenient fluorescence assay to measure the cell's ability to take up fatty acids. Fatty acid substrate can enter the cell through the fatty acid transporter on the cell membrane, and the ability of cells to take up fatty acid can be detected by fluorescence microscopy, fluorescence microplate reader, flow cytometry and other instruments to detect the fluorescence intensity. |
Sample Type | Cell |
Detection Method | Fluorometric method |
Detection Instrument | Fluorescence microplate reader (Ex/Em=485 nm/515 nm), Fluorescence microscope, Flow cytometry |
Research Area | Glycolysis And Lipid Metabolism , Energy Metabolism |
Other Reagents Required | DMSO |
Storage | This product can be stored at -20°C for 12 months with shading light. |
Valid Period | 12 months |
Precision | inter-assay CV: 4.9-12.7% | intra-assay CV: 1.6-3.2% |
Assay Time | 1 h |
Other Clones
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Other Formats
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Biotin
Chromogenic Biotin
Cyanine 3
Cyanine 5
Cyanine 5.5
Cyanine 7
Elab Fluor® Violet 450
Elab Fluor®430
Elab Fluor®488
Elab Fluor®568
Elab Fluor®594
Elab Fluor®647
Elab Fluor®680
Elab Fluor®700
Elab Fluor®750
Elab Fluor®Red 780
Elab Fluor®Violet 450
FITC
HRP
NHS-Biotin
NHS-LC-LC-Biotin
Sulfo-NHS-Biotin
Sulfo-NHS-LC-LC-Biotin
Unconjugated
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