FITC-conjugated Flag-Tag Monoclonal Antibody (AN00299C)

For research use only.
Verified Samples |
Verified Samples in FCM: 293F cells were transfected with a tag expression plasmid to prepare an overexpression cell model |
Dilution | FCM 0.2 μg per 106 cells in a 100 μL suspension |
Isotype | IgG2a |
Host | Mouse |
Reactivity | All |
Applications | FCM |
Clonality | Recombinant |
Immunogen | DYKDDDDK tag fusion protein |
Abbre | Flag Tag |
Synonyms | DDDDK epitope tag, DYKDDDDK epitope tag |
Concentration | 1 mg/mL |
Buffer | PBS with 0.05% proclin 300 and 0.1% gelatin,pH7.4 |
Purification Method | Protein A/G Purification |
Research Areas | Tags and Cell Markers, Microbiology, Immunology, Neuroscience |
Clone No. | 3H9 |
Conjugation | FITC |
Storage | Store at 4°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light. |
Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
Amax | 495nm |
Emax | 525nm |
background | FLAG-tag is a polypeptide protein tag that can be added to a protein using recombinant DNA technology,having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. This antibody detects FLAG tag in most applications. |
Other Clones
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