Freezing Medium (Serum-free & animal origin-free) (PB180438)
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For research use only.
| Product Introduction |
In order to preserve the biological activity of cells in vitro, cells must be cryopreserved for a long time and then revived and cultured when needed. Currently, the most commonly used technique for cell freezing is liquid nitrogen cryopreservation, which primarily involves adding an appropriate amount of protectant to slowly cool the cells to a specified temperature range, thereby protecting the cells. The Serum-free Cell Freezing Medium is a specialized cryopreservation product developed by Procell for use with hundreds of cell types, continuously optimizing experimental conditions for cell cryopreservation and resuscitation in long-term cell research. The product contains a cell sedimentation stabilizer, which can delay the sedimentation rate of cells during the cryopreservation process, preventing cells from squeezing each other and affecting the effectiveness of cell cryopreservation. In addition, a variety of cryoprotectants, such as cell membrane protectors, permeable intracellular membrane protectors, and non-permeable cell protectors, are added. These components combine with water molecules in the solution to hydrate and weaken the crystallization process of water, increasing the viscosity of the solution and reducing the formation of ice crystals, which can greatly reduce the damage of ice crystals to cells during cryopreservation, and effectively improve the survival rate of cells after resuscitation. The product formula is clear, does not contain serum or animal-derived protein, which can reduce contamination risk from bacteria, viruses, and mycoplasma, and ensure the safety of frozen cells. It is not only suitable for conventional cell lines, primary cells, but also serum-free culture cells and protein-expressing cells. Compared with traditional cryopreservation media, there is no need for tedious and time-consuming gradient freezing steps, and no need for expensive program cooling equipment. Cells can be directly resuspended and stored long-term in a -80°C ultra-low temperature freezer, but it is essential to ensure the stability of the freezer's temperature. For more prolonged and stable cell storage, frozen cells can be placed in a -80°C ultra-low temperature freezer overnight (>16 hours) before transferring them to a liquid nitrogen tank for long-term storage. |
| Format | Liquid |
| Mycoplasmal Detection | Negative |
| pH | 7.4-7.6 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Endotoxin Level (EU/mL) | < 3 |
| Storage | This product can be stored at 2-8℃ for 12 months. |
| Shipping | Ice bag |
| Expiration Date | 12 months |
| Matters Need Attention |
1. This product is only used for scientific research or further research, not for diagnosis and treatment. 2. This product has undergone triple 0.1 μm filtration sterilization. Maintain aseptic techniques during use to avoid contamination. 3. For your safety and health, please wear a lab coat and disposable gloves when operating. 4. After aliquoting cryopreserved cells into cryovials, minimize the time they are stored at room temperature or 4℃, and quickly transfer them to an ultra-low temperature freezer set at -80℃, ensuring the stability of the freezer's temperature. 5. Divide the aforementioned cell suspension into sterile cryopreservation tubes at a volume of 0.5 mL or 1.0 mL, tighten the tube caps, and make proper markings. 6. To preserve cells for a longer and more stable period, freeze the cryopreserved cells in a -80℃ ultra-low temperature refrigerator overnight (>16 hours) before transferring them to a liquid nitrogen tank for long-term storage. 7. When cryopreserving cells, please tighten the cap of the cryovial to prevent liquid nitrogen from seeping in, which could lead to the risk of the cryovial cracking during cell revival. 8. For sensitive cell lines, precious cell lines, primary cell samples, etc., it is recommended that you conduct at least one week of cryopreservation testing on the cells before use to confirm there are no issues before proceeding with formal cryopreservation. |
| System Certification |
GMP Production and Quality System The company complies with cGMP requirements, holds the NMPA medical device registration certificate, and is certified under ISO 13485 and ISO 9001 standards. Among its products, the company's Freezing Medium (Serum-free & animal origin-free) complies with ISO 13485, ISO 9001. |
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