Fructose-6-Phosphate (F-6-P) Fluorometric Assay Kit (E-BC-F087)
For research use only.
| Detection Principle |
Fructose-6-phosphate (F-6-P) is a six-carbon sugar formed by esterification of fructose and phosphate. It is an important intermediate in glycolysis and gluconeogenesis pathways. As the most basic metabolic substances in the metabolic process of living bodies and cells, it is of great significance to study them quantitatively. Enzyme catalyze F-6-P to produce a specific product, which can react with chromogenic agent to produce the fluorescent substance. Detect the fluorescent intensity of fluorescent substance at the excitation wavelength of 535 nm and the emission wavelength of 587 nm. |
| Sample Type | Animal and plant tissue,Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Research Area | Glycolysis And Lipid Metabolism |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 1.06 μmol/L |
| Detection Range | 1.06-35.00 μmol/L |
| Precision | inter-assay CV: 5.7-9.8% | intra-assay CV: 4.6-5.0% |
| Sample Volume | 40 μL(tissue/cell homogenate) |
| Assay Time | 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 2-4 |
| 10% Mouse heart tissue homogenate | 3-6 |
| 10% Mouse kidney tissue homogenate | 2-4 |
| 10% Mouse lung tissue homogenate | 3-6 |
| 10% Orange tissue homogenate | 2-5 |
| 10% Orange peel tissue homogenate | 1 |
| 10% Epipremnum aureum tissue homogenate | 2-5 |
| 1×10^6 Jurkat cells | 1 |
| 1×10^6 293T cells | 1 |
| 1×10^6 Hela cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
Other Clones
{{antibodyDetailsPage.numTotal}} Results
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
Other Formats
{{formatDetailsPage.numTotal}} Results
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
-
IF：{{item.impact}}
Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}Reactivity：{{item.species}}
Sample Type：{{item.organization}}
-
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}
