Fructose Colorimetric Assay Kit (E-BC-K134-M)
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For research use only.
| Detection Principle | Fructose is one of the most common hexaketose sugars, which is abundant as a monosaccharide in free form in fruits and honey. A large number of epidemiological data and experimental studies have shown that excessive fructose intake may be an important factor in the increased incidence of metabolic diseases. Fructose can interact with the matrix solution to form a specific product, which has a maximum absorption peak at 285 nm. |
| Sample Type | Serum,Plasma,Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (265-305 nm, optimum wavelength: 285 nm) |
| Research Area | Plant Physiology Series , Metabolic Diseases |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.2 mmol/L |
| Detection Range | 0.2-22.2 mmol/L |
| Precision | inter-assay CV: 3.2-5.3% | intra-assay CV: 2.7-3.1% |
| Sample Volume | 25 μL |
| Assay Time | 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Watermelon pulp tissue homogenization | 2-3 |
| 10% Mango pulp tissue homogenization | 2-3 |
| 10% Cantaloupe pulp tissue homogenization | 2-3 |
| 10% Grape pulp tissue homogenization | 4-5 |
| 10% Orange pulp tissue homogenization | 2-3 |
| 10% Pineapple pulp tissue homogenization | 2-3 |
| 10% broad bean tissue homogenization | 2-3 |
| 10% jackfruit pulp tissue homogenization | 10-15 |
| 10% carrot tissue homogenization | 2-3 |
| 10% banana pulp tissue homogenization | 3-5 |
| Mouse serum | 1 |
| Human serum | 1 |
| Rabbit serum | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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