α-Galactosidase (α-Gal) Acitivity Assay Kit (E-BC-K630-M)
For research use only.
| Detection Principle | α-Galactosidase (α-Gal) catalyzes the substances produced in the glycolysis process of animal and plant tissues to form the final product, which has a maximum absorption peak at 405 nm. |
| Sample Type | Animal tissue,Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (395-415 nm, optimum wavelength: 405 nm) |
| Research Area | Glycolysis And Lipid Metabolism |
| Other Reagents Required | Double distilled water |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.02 U/L |
| Detection Range | 0.02-29.06 U/L |
| Precision | inter-assay CV: 4.0-8.0% | intra-assay CV: 1.0-3.0% |
| Sample Volume | 20 μL(tissue homogenate) |
| Assay Time | 1 h 15 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Sweet potato tissue homogenate | 1 |
| 10% Wood mushroom tissue homogenate | 1 |
| 10% Flammulina velutipes tissue homogenate | 1 |
| 10% Mouse liver tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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