Galectin 3 Polyclonal Antibody (D-AB-10120L)
For research use only.
| Verified Samples |
Verified Samples in WB: Human MCF-7, Mouse NIH/3T3, Mouse Raw264.7 Verified Samples in IHC: Human colon cancer, Human stomach cancer |
| Dilution | WB 1:500-1:2000, IHC 1:150-1:300 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Recombinant Human LGALS3 protein expressed by E.coli |
| Abbre | Galectin |
| Synonyms | 35 kDa lectin, CBP 35, CBP35, Carbohydrate binding protein 35, Carbohydrate-binding protein 35, GAL3, GALBP, GALIG, GBP, Gal-3, Galactose-specific lectin 3, Galactoside-binding protein, Galectin 3 internal gene, Galectin-3, Galectin3, IgE binding protein, IgE-bi, included |
| Swissprot | |
| Calculated MW | 26 kDa |
| Observed MW |
26 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Antigen Affinity Purification |
| Research Areas | Cell Biology, Epigenetics and Nuclear Signaling, Stem Cells, Cancer |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides . Galectin-3 contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers. Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell,being present on the cell surface or in the extracellular space. Galectin-3 is involved in various biological processes including cell growth,adhesion,differentiation,apoptosis,angiogenesis,immune response,neoplastic transformation and metastasis. |
Other Clones
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Other Formats
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Unconjugated
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