gamma Tubulin Monoclonal Antibody (E-AB-22137)
For research use only.
| Verified Samples |
Verified Samples in WB: Jurkat, Hela, Mouse brain, Rat brain Verified Samples in IHC: Human colon carcinoma, Human breast carcinoma |
| Dilution | WB 1:1000-2000, IHC 1:100-200 |
| Isotype | IgG |
| Host | Mouse |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC-p |
| Clonality | Monoclonal |
| Immunogen | Synthetic Peptide of Gamma Tubulin |
| Abbre | Gamma Tubulin Mouse (6C12) |
| Synonyms | GCP-1, Gamma-1-tubulin, Gamma-tubulin complex component 1, TBG1, TUBG, TUBG1, TUBGCP1, Tubulin, Tubulin gamma-1 chain, gamma 1, gamma polypeptide |
| Swissprot | |
| Observed MW |
50 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm>cytoskeleton>centrosome. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
| Purification Method | Protein A purification |
| Research Areas | Signal Transduction |
| Clone No. | 3E4 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7. TUBG1 (Tubulin Gamma 1) is a Protein Coding gene. Diseases associated with TUBG1 include Cortical Dysplasia, Complex, With Other Brain Malformations 4 and Reducing Body Myopathy. Among its related pathways are Regulation of PLK1 Activity at G2/M Transition and Sertoli-Sertoli Cell Junction Dynamics. GO annotations related to this gene include GTP binding and structural constituent of cytoskeleton. An important paralog of this gene is TUBG2. |
Other Clones
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Other Formats
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Unconjugated
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