General Freezing Medium (PB180436)
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For research use only.
| Product Introduction |
Cryopreservation is the main method of cell preservation, which is of great significance for cell preservation, introduction, culture and experimental research. In order to protect cells from freezing damage, cryoprotectants are often added. Currently, the most widely used osmotic protectant is DMSO (dimethyl sulfoxide). Its protective mechanism is to penetrate into cells before the cell freezing solution is completely solidified, create a certain molar concentration inside and outside the cells, and reduce the concentration of electrolytes in the unfrozen solution inside and outside the cells. In this way, the cells are protected from the damage of high electrolyte concentration, and at the same time, the water in the cells will not leak excessively, to avoid excessive dehydration and shrinkage of cells. However, DMSO has a greater cytotoxic effect at room temperature, and at higher concentrations it has a greater cytotoxic effect, especially on sensitive cells such as hybridoma cells. In general, DMSO has a good effect when the final concentration is between 5% and 15%. The General Freezing Medium produced by Procell is composed of DMSO, FBS and DMEM/F12 basal medium. The product has been verified by nearly one thousand kinds of cells. It is suitable for the cryopreservation of various mammalian primary cells, passaged cell lines and hybridoma cells. The performance is stable and it's easy to use. More importantly, the cell survival rate is high. |
| Format | Liquid |
| Mycoplasmal Detection | Negative |
| pH | 7.8-8.0 |
| Green Features | Sustainable packaging |
| Endotoxin Level (EU/mL) | < 3 |
| Storage | This product can be stored at 2-8℃ for 3 months or -20°C for 24 months with shading light. |
| Shipping | Ice bag |
| Expiration Date | 24 months |
| Matters Need Attention |
1. This product has undergone triple 0.22 μm filtration sterilization. Maintain aseptic techniques during use to avoid contamination. 2. Thaw the product at 2-8°C, mix thoroughly before use, and avoid repeated freeze-thaw cycles. For small-volume usage, aliquot before freezing. 3. Avoid prolonged storage at room temperature. After initial thawing, the product can be stored at 2-8°C in the dark for up to 3 months. 4. If slight protein precipitation occurs, the product can be used directly without affecting cryopreservation efficacy. Alternatively, remove precipitates by centrifugation before use. 5. Wear a lab coat and disposable gloves during operation to ensure personal safety. 6. After aliquoting cells into cryovials, minimize their exposure to room temperature or 4°C. Transfer them promptly to a -80°C ultra-low temperature freezer, and ensure the freezer maintains stable temperature conditions. 7. For long-term storage, you should store the cells overnight (>16 hours) in the -80°C ultra-low temperature freezer after perform programmed cooling of cryopreserved cells. Subsequently, transfer the cryovials to a liquid nitrogen tank for long-term preservation. 8. Ensure the cap of the cryovial is tightly sealed before freezing to prevent liquid nitrogen infiltration. Leakage of liquid nitrogen may cause cryovial explosion during cell thawing. 9. For special cells (e.g., sensitive cell lines, rare/valuable cell lines, primary cells), conduct a preliminary cryopreservation test for at least 1 week prior to formal freezing. Proceed to full-scale cryopreservation only after confirming successful cell viability and functionality. 10. This product is intended only for research or further investigational use. It is not approved for diagnostic, therapeutic, clinical, or any other applications. |
| System Certification |
GMP Production and Quality System The company complies with cGMP requirements, holds the NMPA medical device registration certificate, and is certified under ISO 13485 and ISO 9001 standards. Among its products, the company's General Freezing Medium complies with ISO 13485, ISO 9001. |
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