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For research use only.

Verified Samples Verified Samples in WB: Rat brain
Verified Samples in IHC: Mouse kidney
Verified Samples in IF: Mouse brain
Dilution WB 1:500-1:5000,  IHC 1:50-1:300,  IF 1:100-1:300
Isotype IgG
Host Mouse
Reactivity Mouse,  Rat
Applications WB,  IHC-p,  IF
Clonality Monoclonal
Immunogen Synthetic Peptide
Abbre GFAP
Synonyms ALXDRD,  FLJ42474,  FLJ45472,  GFAP,  Glial fibrillary acidic protein,  Intermediate filament protein,  cb345,  etID36982.3,  gfapl,  wu:fb34h11,  wu:fk42c12,  xx:af506734,  zgc:110485
Swissprot
Calculated MW 50 kDa
Observed MW 45 kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cytoplasm
Tissue Specificity Expressed in cells lacking fibronectin
Concentration 1 mg/mL
Buffer Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol.
Purification Method Protein A purification
Research Areas Cancer,  Neuroscience,  Signal Transduction,  Stem Cells,  Tags and Cell Markers
Clone No. 3H1
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Other Clones

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Unconjugated

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