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For research use only.

Verified Samples Verified Samples in WB: U251, C6, Mouse brain, Rat brain
Verified Samples in IHC: Mouse brain, Rat brain, Human cerebellum
Verified Samples in IF: Mouse brain
Dilution WB 1:1000-1:3000,  IHC 1:300-1:800,  IF 1:200-1:500
Isotype IgG
Host Rabbit
Reactivity Human,  Mouse,  Rat
Applications WB,  IHC,  IF
Clonality Polyclonal
Immunogen KLH conjugated Synthetic peptide corresponding to Mouse GFAP
Abbre GFAP
Synonyms ALXDRD,  FLJ42474,  FLJ45472,  GFAP,  Glial fibrillary acidic protein,  Intermediate filament protein,  cb345,  etID36982.3,  gfapl,  wu:fb34h11,  wu:fk42c12,  xx:af506734,  zgc:110485
Swissprot
Calculated MW 49 kDa
Observed MW 49 kDa

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cytoplasm
Concentration 1.2 mg/mL
Buffer PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH7.4
Purification Method Affinity purification
Research Areas Cancer,  Neuroscience,  Signal Transduction,  Stem Cells,  Tags and Cell Markers
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background GFAP (Glial fibrillary acidic protein ),an intermediate-filament (IF) protein ,is specifically expressed in cells of astroglial lineage and is widely used to mark the astroglia in the brain. It is also used as a marker for intracranial and intraspinal tumors arising from astrocytes.
Other Clones

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Unconjugated

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