Glucose (GLU) Fluorometric Assay Kit (E-BC-F037)
For research use only.
| Detection Principle | Glucose oxidase can catalyze the oxidation of glucose into gluconic acid and produce hydrogen peroxide. In the presence of peroxidase, hydrogen peroxide reacts with the non-fluorescent substance to form fluorescent substance. The glucose content can be calculated indirectly by measuring the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 590 nm. |
| Synonyms | GLU |
| Sample Type | Serum,Plasma,Urine,Saliva,Milk,Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=535 nm/590 nm) |
| Research Area | Metabolic Diseases , Glycolysis And Lipid Metabolism , Disulfidptosis , Energy Metabolism |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.1 μmol/L |
| Detection Range | 0.1-20 μmol/L |
| Precision | inter-assay CV: 2.8% | intra-assay CV: 1.7% |
| Assay Time | 25 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human plasma | 300-600 |
| Human serum | 300-600 |
| Chicken serum | 600-1000 |
| Human urine | 1 |
| Human milk | 400-600 |
| Saliva | 3-5 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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