Glutamate Oxidase (GLOD) Activity Fluorometric Assay Kit (E-BC-F094)
For research use only.
| Detection Principle |
L-glutamate oxidase (GLOD) is an L-amino acid oxidase with flavin adenine dinucleotide (FAD) as the coenzyme. However, due to its own presence of a non-covalently bound FAD, Therefore, the catalytic reaction can be completed without the addition of exogenous cofactor FAD, and L-glutamic acid can be deaminated to generate α-ketoglutaric acid, ammonia and hydrogen peroxide. L-glutamate oxidase has high specificity and affinity for reaction substrates, mild reaction conditions and high catalytic efficiency, and is widely used in the food, industrial fermentation and pharmaceutical industries. The detection principle of this kit: Glutamate oxidase oxidizes the substrate, and the resulting substance reacts with the chromogenic reagent to produce fluorescence. The fluorescence value is detected at the excitation wavelength of 535 nm and the emission wavelength of 587 nm. The enzyme activity is calculated based on the fluorescence value of the standard. |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Research Area | Energy Metabolism |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.1 mU/mL |
| Detection Range | 0.1-17.4 mU/mL |
| Precision | inter-assay CV: 4.6%-10.0% | intra-assay CV: 3.3%-5.0% |
| Sample Volume | 20 μL |
| Assay Time | 30 min |
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