Glutamic Acid Colorimetric Assay Kit (E-BC-K118-S)

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All	Size	Price	Qty
	100Assays	$ 280.00
	50Assays	$ 200.00

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Detection Principle	Glutamic acid can react with NAD⁺ under the catalysis of glutamate dehydrogenase to produce α-ketoglutaric acid, NADH and NH⁴⁺. NADH has the maximum absorption at 340 nm. And glutamic acid content can be calculated by measuring the change of NADH.
Sample Type	Serum,Plasma,Animal tissue,Cell,Cell culture supernatant
Detection Method	Colorimetric method
Detection Instrument	Spectrophotometer (340 nm)
Research Area	Amino Acids And Proteins , Ferroptosis
Other Reagents Required	Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4)
Storage	This product can be stored at 2-8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.
Valid Period	12 months
Sensitivity	4.00 μmol/L
Detection Range	4.00-450 μmol/L
Precision	inter-assay CV: 2.9% \| intra-assay CV: 2.2%
Assay Time	1 h

Cat.No.	Product Name	Sizes
E-BC-K130-S	Pyruvic Acid Colorimetric Assay Kit	100Assays , 50Assays
E-BC-K177-S	Proline (Pro) Colorimetric Assay Kit	100Assays , 50Assays

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
Human serum	1
Mouse serum	1
10% Rat liver tissue homogenate	1
10% Rat kidney tissue homogenate	1
10% Rat brain tissue homogenate	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.