Glutamine (Gln) Colorimetric Assay Kit (E-BC-K853-M)
For research use only.
| Detection Principle | Gln is hydrolyzed to produce glutamic acid under the action of glutaminase. Glutamic acid is further catalyzed by glutamic acid dehydrogenase. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The content of Gln can be calculated by measuring the change of absorbance value at 450 nm. |
| Synonyms | Gln |
| Sample Type | Serum,Plasma,Tissue,Cell,Cell culture supernatant |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (440-460 nm, optimum wavelength: 450 nm) |
| Research Area | Amino Acids And Proteins , Cuproptosis , Energy Metabolism |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.036 mmol/L |
| Detection Range | 0.036–2.0 mmol/L |
| Precision | inter-assay CV: 4.7% | intra-assay CV: 3.2% |
| Sample Volume | 50 μL |
| Assay Time | 1 h 10 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Rat liver tissue homogenate | 1 |
| 10% Mouse lung tissue homogenate | 1 |
| 10% Rat brain tissue homogenate | 1 |
| 10% Mouse liver tissue homogenate | 1 |
| 10% Rat spleen tissue homogenate | 3-5 |
| 10% Epipremnum aureum tissue homogenate | 3-5 |
| 1 × 10^6 THP-1 cell | 1 |
| 2 × 10^6 Molt-4 cell | 1 |
| Rat plasma | 1 |
| Mouse serum | 1 |
| Mouse plasma | 1 |
| Human serum | 2-3 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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