Glutamine (Gln) Colorimetric Assay Kit (E-BC-K853-M)

For research use only.
Detection Principle | Gln is hydrolyzed to produce glutamic acid under the action of glutaminase. Glutamic acid is further catalyzed by glutamic acid dehydrogenase. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The content of Gln can be calculated by measuring the change of absorbance value at 450 nm. |
Synonyms | Gln |
Sample Type | Serum,Plasma,Tissue,Cell,Cell culture supernatant |
Detection Method | Colorimetric method |
Detection Instrument | Microplate reader (440-460 nm, optimum wavelength: 450 nm) |
Research Area | Amino Acids And Proteins , Cuproptosis , Energy Metabolism |
Other Reagents Required | Normal saline (0.9% NaCl) |
Storage | This product can be stored at -20°C for 12 months with shading light. |
Valid Period | 12 months |
Sensitivity | 0.036 mmol/L |
Detection Range | 0.036–2.0 mmol/L |
Precision | inter-assay CV: 4.7% | intra-assay CV: 3.2% |
Assay Time | 1 h 10 min |
The recommended dilution factor for different samples is as follows (for reference only):
Sample Type | Dilution Factor |
---|---|
10% Rat liver tissue homogenate | 1 |
10% Mouse lung tissue homogenate | 1 |
10% Rat brain tissue homogenate | 1 |
10% Mouse liver tissue homogenate | 1 |
10% Rat spleen tissue homogenate | 3-5 |
10% Epipremnum aureum tissue homogenate | 3-5 |
1 × 10^6 THP-1 cell | 1 |
2 × 10^6 Molt-4 cell | 1 |
Rat plasma | 1 |
Mouse serum | 1 |
Mouse plasma | 1 |
Human serum | 2-3 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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