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All Size Price Qty
96T $ 440.00
48T $ 295.00
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For research use only.

Detection Principle Gln is hydrolyzed to produce glutamic acid under the action of glutaminase. Glutamic acid is further catalyzed by glutamic acid dehydrogenase. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The content of Gln can be calculated by measuring the change of absorbance value at 450 nm.
Synonyms Gln
Sample Type Serum,Plasma,Tissue,Cell,Cell culture supernatant
Detection Method Colorimetric method
Detection Instrument Microplate reader (440-460 nm, optimum wavelength: 450 nm)
Research Area Amino Acids And Proteins ,   Cuproptosis ,  Energy Metabolism
Other Reagents Required Normal saline (0.9% NaCl)
Storage This product can be stored at -20°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.036 mmol/L
Detection Range 0.036–2.0 mmol/L
Precision inter-assay CV: 4.7% | intra-assay CV: 3.2%
Assay Time 1 h 10 min
Cat.No. Product Name Sizes
E-BC-K660-M Glutaminase (GLS) Activity Assay Kit 96T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
10% Rat liver tissue homogenate 1
10% Mouse lung tissue homogenate 1
10% Rat brain tissue homogenate 1
10% Mouse liver tissue homogenate 1
10% Rat spleen tissue homogenate 3-5
10% Epipremnum aureum tissue homogenate 3-5
1 × 10^6 THP-1 cell 1
2 × 10^6 Molt-4 cell 1
Rat plasma 1
Mouse serum 1
Mouse plasma 1
Human serum 2-3

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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