γ-Glutamyl Transferase (GGT/γ-GT) Activity Assay Kit (E-BC-K126-M)
For research use only.
| Detection Principle | γ-GT catalyzes the transfer of gamma glutamyl group from glutamyl p-nitroaniline to N-glycyl glycine to produce p-nitroaniline, which has characteristic absorption peak at 405 nm. The activity of γ-GT can be calculated according to the changing rate of absorbance value. |
| Synonyms | GGT;γ-GT |
| Sample Type | Serum,Plasma,Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (405 nm) |
| Research Area | Liver And Renal Function |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.88 U/L |
| Detection Range | 0.88-399.4 U/L |
| Precision | inter-assay CV: 6.2% | intra-assay CV: 4.2% |
| Sample Volume | 25 μL |
| Assay Time | 20 min |
Note: Data for this module come from published literature that cites this product, displaying other products used together in the same research paper.
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Mouse serum | 1 |
| Human serum | 1 |
| Rat serum | 1 |
| Dog serum | 1 |
| Human plasma | 1 |
| Horse serum | 1 |
| Porcine serum | 1 |
| Human hydrothorax | 1 |
| 10% Mouse liver tissue homogenate | 1 |
| 10% Mouse heart tissue homogenate | 1 |
| 10% Rat spleen tissue homogenate | 1 |
| 10% Rat lung tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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