Glycerol Kinase (GK) Acitivity Colorimetric Assay Kit (E-BC-K791-M)
For research use only.
| Detection Principle | Glycerol kinase (GK) is a rate-limiting enzyme in glycerol metabolism, and the lack of this enzyme directly results in the inability of cells to utilize glycerol. This kit produces hydrogen peroxide under the action of glycerophosphate oxidase. The reaction between hydrogen peroxide and the chromogenic agent has the maximum absorption peak at 555 nm. The enzyme activity of GK can be determined by measuring the increase rate at 555 nm. |
| Sample Type | Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (545-656 nm, optimum wavelength: 555 nm) |
| Research Area | Glycolysis And Lipid Metabolism , Energy Metabolism |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 9.28 U/L |
| Detection Range | 9.28-170.00 U/L |
| Precision | inter-assay CV: 6.5-8.0% | intra-assay CV: 3.0-3.6% |
| Sample Volume | 10 μL(tissue homogenate) |
| Assay Time | 50 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenization | 1 |
| 10% Mouse kidney tissue homogenization | 1 |
| 10% Mouse small intestine tissue homogenization | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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