Glycogen Fluorometric Assay Kit (Glycosidase Method) (E-BC-F171)
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For research use only.
| Detection Principle | Glycogen is converted into glucose under the action of starch glycosidase. Glucose is then catalyzed by glucose oxidase to produce hydrogen peroxide. Under the action of peroxidase, the non-fluorescent probe is oxidized to a fluorescent substance, and its fluorescence intensity is related to the content of glycogen. |
| Sample Type | Animal tissue,Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Research Area | Glycolysis And Lipid Metabolism,Energy Metabolism |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.014 μg/mL |
| Detection Range | 0.014-4 μg/mL |
| Precision | inter-assay CV: 3.2%-6.0% | intra-assay CV: 3.7%-5.2% |
| Sample Volume | 50 μL(tissue homogenate/cell homogenate) |
| Assay Time | 1 h 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 200-400 |
| 10% Mouse muscle tissue homogenate | 5-10 |
| 1×10^6 HepG2 cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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