Glycogen Fluorometric Assay Kit (Glycosidase Method) (E-BC-F171)

Name: Glycogen Fluorometric Assay Kit (Glycosidase Method)
Brand: Elabscience
SKU: E-BC-F171
Price: 320.0 USD
Availability: InStock

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Glycogen Fluorometric Assay Kit (Glycosidase Method) - 1

All	Size	Price	Qty
	96T	$ 320.00	- +

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Detection Principle	Glycogen is converted into glucose under the action of starch glycosidase. Glucose is then catalyzed by glucose oxidase to produce hydrogen peroxide. Under the action of peroxidase, the non-fluorescent probe is oxidized to a fluorescent substance, and its fluorescence intensity is related to the content of glycogen.
Sample Type	Animal tissue,Cell
Detection Method	Fluorometric method
Detection Instrument	Fluorescence microplate reader (Ex/Em=535 nm/587 nm)
Research Area	Glycolysis And Lipid Metabolism,Energy Metabolism
Storage	This product can be stored at -20°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	0.014 μg/mL
Detection Range	0.014-4 μg/mL
Precision	inter-assay CV: 3.2%-6.0% \| intra-assay CV: 3.7%-5.2%
Sample Volume	50 μL(tissue homogenate/cell homogenate)
Assay Time	1 h 30 min

Cat.No.	Product Name	Sizes
E-BC-F037	Glucose (GLU) Fluorometric Assay Kit	96T , 48T
E-BC-K234-M	Glucose (Glu) Colorimetric Assay Kit (GOD-POD Method)	500Assays , 96T , 48T
E-BC-K234-S	Glucose (Glu) Colorimetric Assay Kit (GOD-POD Method)	100Assays , 50Assays
E-BC-K520-M	Glucose Oxidase (GOD) Activity Assay Kit	96T , 48T
E-BC-K822-M	β-Glucosidase (β-GC) Activity Assay Kit	96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
10% Mouse liver tissue homogenate	200-400
10% Mouse muscle tissue homogenate	5-10
1×10^6 HepG2 cells	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.