For research use only.
| Verified Samples |
Verified Samples in WB: Human Jurkat, Human K562, Rat kidney, Mouse liver, Rat brain Verified Samples in IHC: Human lung, Human kidney |
| Dilution | WB 1:500-1:2000, IHC 1:50-1:150 |
| Clonality | Polyclonal |
| Immunogen | Recombinant Human GSTP1 protein expressed by E.coli |
| Abbre | GSTp1 |
| Synonyms | DFN7, Deafness X-linked 7, FAEES3, Fatty Acid Ethyl Ester Synthase III, GST class-pi, GST3, GSTP, Glutathione S Transferase 3, Gstp1, PI, X linked 7, Deafness |
| Swissprot | |
| Calculated MW | 23 kDa |
| Observed MW |
26 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.02% sodium azide,1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Affinity purification |
| Research Areas | Cardiovascular, Neuroscience, Metabolism |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. |
Other Clones
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