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For research use only.

Verified Samples Verified Samples in WB: SW480, 293T, HepG2, HL-60, A549
Verified Samples in IF: A549
Dilution WB 1:500-1:2000,  IF 1:50-1:200
Isotype IgG
Host Rabbit
Reactivity Human
Applications WB,  IF
Clonality Polyclonal
Immunogen Recombinant fusion protein of human GTF2F2 (NP_004119.1).
Synonyms BTF4,  GTF2F2,  RAP30,  TF2F2,  TFIIF
Swissprot
Calculated MW 28 kDa
Observed MW 29 kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Nucleus.
Concentration 1 mg/mL
Buffer Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol.
Purification Method Affinity purification
Research Areas Cancer,  Epigenetics and Nuclear Signaling
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background GTF2F2 (General Transcription Factor IIF Subunit 2) is a Protein Coding gene. Among its related pathways are RNA Polymerase II Transcription Initiation And Promoter Clearance and Signaling by FGFR2 in disease. Gene Ontology (GO) annotations related to this gene include helicase activity and ATP-dependent helicase activity.
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Unconjugated

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