HDL, LDL/VLDL and TC Fluorometric Assay Kit (E-BC-F034)
For research use only.
| Detection Principle |
High-density lipoprotein (HDL), low-density lipoprotein (LDL) and very low-density lipoprotein (VLDL) are core indicators for evaluating blood lipid status, among which high LDL levels and low HDL levels are closely associated with an increased risk of cardiovascular diseases. HDL is known as "good cholesterol" and has anti-atherosclerotic effects; LDL is known as "bad cholesterol" and tends to deposit on blood vessel walls to form plaques when in excess. This kit uses a selective separation reagent to separate HDL from LDL/VLDL. The separated cholesterol is catalyzed by cholesterol esterase and cholesterol oxidase to generate 4-cholesten-3-one and hydrogen peroxide. In the presence of peroxidase (POD), hydrogen peroxide reacts with a non-fluorescent probe to generate a strongly red-fluorescent resorufin analog. The fluorescence intensity is measured at an excitation wavelength of 535 nm and an emission wavelength of 587 nm, and is proportional to the cholesterol concentration, allowing HDL and LDL/VLDL contents to be calculated separately. Meanwhile, total cholesterol (TC) can also be directly measured without separation. |
| Sample Type | Serum,Plasma,Animal tissue,Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Research Area | Lipid Profile |
| Other Reagents Required | PBS (0.01 M, pH 7.4), isopropanol (AR), double distilled water |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 2.52 μmol/L |
| Detection Range | 2.52-260 μmol/L |
| Precision | inter-assay CV: 3.1%-9.3% | intra-assay CV: 4.3%-5.4% |
| Sample Volume | 10 μL |
| Assay Time | 1 h 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 2-3 |
| Mouse serum | 2-3 |
| Rat serum | 2-3 |
| Pig serum | 1 |
| Guinea pig serum | 1 |
| Horse serum | 1 |
| 10% Pig liver tissue homogenate | 2-3 |
| 10% Mouse liver tissue homogenate | 1 |
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Mouse spleen tissue homogenate | 1 |
| 10% Mouse heart tissue homogenate | 1 |
| 10% Mouse muscle tissue homogenate | 1 |
| 1×10^6 HeLa cells | 1 |
| 1×10^6 293T cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
-
IF：{{item.impact}}
Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}Reactivity：{{item.species}}
Sample Type：{{item.sample_type}}
-
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}
No files available. Please enter the correct identification code in the search area to query the relevant documents.
| Title | Status | Valid until | Action | |
|---|---|---|---|---|
| {{v.文件名}} | {{v.status_str}} | {{v.timestamp_str}} | Download | / |
*Note：Please download the relevant files within the validity period. After expiration, file preview and download will not be available on the page.
