Hdroxyproline (HYP) Colorimetric Assay Kit (Alkali Hydrolysis Method) (E-BC-K061-M)
For research use only.
| Detection Principle |
Hydroxyproline (HYP) is a derivative of proline, mainly found in collagen. Collagen is the most abundant protein in animals and makes up connective tissues such as skin, bone, cartilage, tendons and ligaments. HYP enhances the crosslinking of collagen fibers through the hydroxyl group in its structure, thus improving the stability and tensile strength of collagen. Since HYP almost only exists in collagen, its content can reflect the synthesis and catabolism of collagen. Therefore, the detection of HYP level can be used as an indicator to monitor collagen metabolism. The detection principle of this kit: the sample is hydrolyzed by the alkali reagent to generate free HYP, and the oxidation product produced under the action of the oxidant reacts with the color agent to be purple red. The substance has an absorption peak at 565 nm, and within a certain range, its absorbance is linear with the concentration. By measuring the absorbance at 565 nm, the HYP content in the sample can be calculated. |
| Sample Type | Serum,Plasma,Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (555-565 nm, optimum wavelength: 565 nm) |
| Research Area | Amino Acids And Proteins |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.036 μg/mL |
| Detection Range | 0.036-5 μg/mL |
| Precision | inter-assay CV: 2.4-3.4% | intra-assay CV: 1.4-2.1% |
| Sample Volume | 1 mL |
| Assay Time | 2 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Rat serum | 1 |
| Rat plasma | 1 |
| Fish scale | 4-10 |
| Fin tissue | 4-10 |
| Fishtail tissue | 4-10 |
| Mouse lung tissue | 1 |
| Mouse muscle tissue | 2-5 |
| Mouse leg bone tissue | 4-10 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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