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For research use only.

Verified Samples Verified Samples in WB: Mouse brain, Mouse liver
Verified Samples in IHC: Human esophagus cancer, Human brain
Dilution WB 1:500-1:2000,  IHC 1:50-1:300
Isotype IgG
Host Rabbit
Reactivity Human,  Mouse
Applications WB,  IHC
Clonality Polyclonal
Immunogen Synthetic peptide of human HIF1AN
Abbre HIF1AN
Synonyms DKFZp762F1811,  FIH 1,  FIH-1,  FIH1,  FLJ20615,  FLJ22027 ,  Factor inhibiting HIF-1,  Factor inhibiting HIF1,  HIF1AN,  HIF1N,  Hyp,  Hypoxia inducible factor 1 alpha inhibitor,  Hypoxia inducible factor 1 alpha subunit inhibitor,  Hypoxia inducible factor asparagine hydroxylase
Swissprot
Calculated MW 40 kDa
Cellular Localization Nucleus.
Concentration 0.6 mg/mL
Buffer Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol.
Purification Method Affinity purification
Research Areas Cancer,  Epigenetics and Nuclear Signaling
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background Factor inhibiting HIF-1 (FIH-1) exists as a homodimer and binds to HIF-1alpha.Specifically, FIH-1 operates as an asparaginyl hydroxylase.It catalyzes the hydroxylation of the beta-carbon of Asparagine residue 803 within the carboxy terminal transactivation domain of HIF-1alpha.This hydroxylation event blocks the association of HIF-1alpha with co-activators.FIH-1 also binds to von Hippel-Lindau (VHL) tumor suppressor protein, which represses transcriptional ac-tivity of HIF-1alpha.In transiently transfected human osteosarcoma cells, FIH-1 localizes to the cytoplasm.
Other Clones

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Unconjugated

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