For research use only.
| Verified Samples |
Verified Samples in WB: 231 Verified Samples in IHC: Human esophagus cancer, Human liver cancer |
| Dilution | WB 1:500-1:2000, IHC 1:30-1:150 |
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide of human HIPK2 |
| Abbre | HIPK2 |
| Synonyms | HIPK 2, HIPK2, Hipk2, Homeodomain interacting protein kinase 2, Homeodomain-interacting protein kinase 2, Nbak1, Nuclear body-associated kinase 1, PRO0593, Sialophorin tail-associated nuclear serine/threonine-protein kinase , Stank, hHIPk 2, hHIPk2 |
| Swissprot | |
| Calculated MW | 131 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus>PML body. Cytoplasm. Concentrated in PML/POD/ND10 nuclear bodies. Small amounts are cytoplasmic. |
| Concentration | 1.1 mg/mL |
| Buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
| Purification Method | Antigen affinity purification |
| Research Areas | Cancer, Cell Biology, Epigenetics and Nuclear Signaling, Signal transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | This gene encodes a conserved serine/threonine kinase that is a member of the homeodomain-interacting protein kinase family. The encoded protein interacts with homeodomain transcription factors and many other transcription factors such as p53, and can function as both a corepressor and a coactivator depending on the transcription factor and its subcellular localization. Multiple transcript variants encoding different isoforms have been found for this gene. |
Other Clones
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