Histamine Colorimetric Assay Kit (E-BC-K893-M)
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For research use only.
| Detection Principle | Histamine is an endogenous active substance that exists widely in nature and is an important mediator involved in inflammatory response and immune damage. The histamine content increases with the degree of spoilage of the organism. Histamine can produce chromogenic substances with chromogenic agents under the action of enzymes, which has a maximum absorption peak at 460 nm. The histamine content of the sample can be calculated by measuring the OD value at 460 nm. |
| Sample Type | Serum,Plasma,Animal tissue,Plant tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (440-480 nm, optimum wavelength: 460 nm) |
| Research Area | Amino Acids And Proteins |
| Other Reagents Required | Double distilled water |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 2.44 μmol/L |
| Detection Range | 2.44-200 μmol/L |
| Precision | inter-assay CV: 1.5-4.2 % | intra-assay CV: 0.5-1.6 % |
| Sample Volume | 40 μL |
| Assay Time | 1 h 10 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Fresh salmon tissue homogenate | 1 |
| 10% Pelteobagrus fulvidraco tissue homogenate | 1 |
| 10% Spanish mackerel tissue homogenate | 1 |
| 10% Spoiled Spanish mackerel tissue homogenate | 1 |
| 10% Thunnini tissue homogenate | 3-6 |
| 10% Spoiled Thunnini tissue homogenate | 4-10 |
| 10% Pepper tissue homogenate | 2-8 |
| 10% Grape flesh tissue homogenate | 1 |
| 10% Garlic tissue homogenate | 1 |
| 10% jujube tissue homogenate | 2-8 |
| Rat serum | 1 |
| Mouse serum | 1 |
| Fetal calf serum | 1 |
| 0.2 × 10^6 Jurkat cell | 1 |
| 0.2 × 10^6 4T1 cell | 1 |
| 0.2 × 10^6 HL-60 cell | 1 |
| 0.2 × 10^6 293T cell | 1 |
| 0.2 × 10^6 CHO cell | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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