HO1/HMOX1 Polyclonal Antibody (AN000380P)
For research use only.
| Verified Samples |
Verified Samples in WB: A549, NIH-3T3 Verified Samples in IHC: Human breast cancer Verified Samples in ELISA: Recombinant Human HO1/HMOX1 protein, Human serum, Human plasma |
| Dilution | WB 1:500-1:1000, IHC 1:500-1:1000, ELISA Capture 2-8 μg/mL, ELISA Detector 0.1-0.4 μg/mL |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, ELISA Capture/Detector |
| Clonality | Polyclonal |
| Immunogen | Recombinant Human HO1/HMOX1 protein expressed by E.coli |
| Abbre | HO1/HMOX1 |
| Synonyms | Heme oxygenase 1, HO-1, HMOX1, HO, HO1, EC:1.14.14.18 |
| Swissprot | |
| Calculated MW | 33 kDa |
| Observed MW |
25 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.2, containing 0.05% proclin 300. |
| Purification Method | Antigen Affinity Purification |
| Research Areas | Neuroscience, Cardiovascular, Signal Transduction, Epigenetics and Nuclear Signaling, Cancer, Metabolism |
| Conjugation | Unconjugated |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
| background | Heme Oxygenase (HO),also known as HMOX,is the rate limiting enzyme in the breakdown of heme into Biliverdin,CO,and iron. There are two major isoforms of this enzyme,HO-1 and HO-2. Both have similar enzymatic activity,however,HO-1 is rapidly inducible,while HO-2 is constitutively expressed. Inducers of HO-1 include cell stressors such as oxidative stress,infection,hypoxia,and cytotoxic agents. The reaction products generated by HO-1 activity are biologically active,and the enzyme has a broad range of putative roles. For instance,Biliverdin is an antioxidant with cytoprotective and anti-inflammatory properties,and CO has the potential to suppress inflammation and apoptosis. Because of these activities,HO-1 has received much attention as a therapeutic target for a range of pathological processes. |
Other Clones
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Unconjugated
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