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HRP-conjugated Lamin B1 Monoclonal Antibody (AN00292HP)

All Size Price Qty
100μL $ 150.00
25μL $ 60.00
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For research use only.

Verified Samples Verified Samples in WB: Raji, HL-60, Hela, SH-SY5Y, THP-1
Dilution WB 1:2500-1:5000
Isotype IgG2b
Host Mouse
Reactivity Human
Applications WB
Clonality Monoclonal
Immunogen Recombinant human Lamin B1 protein by E. Coli
Abbre Lamin B1
Synonyms ADLD,  LMN,  LMN2,  LMNB,  LMNB1,  Lamin-B1,  Lmnb1,  MGC111419,  OTTHUMP00000159218,  lamin B1
Swissprot
Calculated MW 66 kDa
Observed MW 70 kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Nucleus lamina
Concentration 1 mg/mL
Buffer PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method Protein A/G Purification
Research Areas Cancer,  Cell Biology,  Signal Transduction,  Tags and Cell Markers,  Epigenetics and Nuclear Signaling,  Isotype/Loading Controls,  Cardiovascular
Clone No. 7H10
Conjugation HRP
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background Lamins are components of the nuclear lamina,a fibrous layer on the nucleoplasmic side of the inner nuclear membrane,which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
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HRP

Unconjugated

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