HSP60 Polyclonal Antibody (E-AB-70103)
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For research use only.
| Verified Samples |
Verified Samples in WB: U87, U251, SH-SY5Y, RGC-5, RAW264.7, Mouse spleen, Mouse brain, Rat slpeen, Rat brain Verified Samples in IHC: Human colon, Mouse kidney, Rat kidney |
| Dilution | WB 1:2000-1:5000, IHC 1:300-1:800 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse HSP60 |
| Abbre | HSP60 |
| Synonyms | 60 kDa chaperonin, 60 kDa heat shock protein, 60-KD, CH60, CPN60, Chaperonin, Chaperonin 60, GROEL, Heat shock protein 1 (chaperonin), Heat shock protein 60, Heat shock protein 65, fa04a05, heat shock 60kDa protein 1 (chaperonin), heat shock protein fami, mitochondrial |
| Swissprot | |
| Calculated MW | 60 kDa |
| Observed MW |
60 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Mitochondrion |
| Concentration | 1.6 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 1% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Signal Transduction, Tags and Cell Markers |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | HSPD1,also known as HSP60,belongs to the chaperonin family and acts as a chaperone to enhance cell survival under physiological stresses. Hsp60 has been shown to be connected with many aspects of cell functions such as protein folding and assembling of polypeptide chains in mitochondria. Recently it has been reported that HSP60 is associated with apoptosis or inhibition of cancer cell growth. (21822415) |
Other Clones
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Unconjugated
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