Human HPX(Hemopexin) ELISA Kit (E-EL-H5619)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HPX. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HPX and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HPX, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HPX. You can calculate the concentration of Human HPX in the samples by comparing the OD of the samples to the standard curve.

Heme (HPX) is a serum glycoprotein. Through receptor-mediated endocytosis, it is transported to tissues such as the liver in a manner similar to transferrin, and the complete heme is recycled. The affinity between heme and heme binding is the highest among all known proteins. Its function is to remove heme proteins such as? Hemoglobin protects the body from oxidative damage caused by free heme. In addition, hemagglutinin releases the ligands it binds to. Through the internalization of interaction with specific receptors located on the surface of liver cells, it involves the preservation of iron in the body.