Human IL-10 (Interleukin 10) ELISPOT Kit (ESP-H0003)
For research use only.
Elabscience®developed high-sensitivity ELISPOT kit, focusing on cytokine detection, to achieve single-cell level, dynamic immune function detection, ultra-high sensitivity of one per million, intuitive and reliable results.
| Size | 96T / 96T*5 |
| Manual Operation Time | 1 h |
| Assay Time | 3 h 30 min |
| Reacitivity | Human |
| applications | ELISPOT |
| Sample Volume | 100 μL |
| Sample Type | Suspension cell |
| Cross Reactivity | This kit recognizes Human IL-10 in samples.No significant cross-reactivity or interference between Human IL-10 and analogues was observed. |
| Usage | This ELISPOT kit is designed for the detection of the frequency of human IL-10 secreting cells. |
| Precision | Both intra-CV and inter-CV are < 10%. |
| Storage | 2-8℃ |
| Expiration Date | 12 months |
| Precision | Both intra-CV and inter-CV are < 10%. |
| Background | Interleukin 10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans, interleukin 10 is encoded by the IL10 gene. IL-10 signals through a receptor complex consisting of two IL-10 receptor-1 and two IL-10 receptor-2 proteins. Consequently, the functional receptor consists of four IL-10 receptor molecules. IL-10 binding induces STAT3 signalling via the phosphorylation of the cytoplasmic tails of IL-10 receptor 1 + IL-10 receptor 2 by JAK1 and Tyk2 respectively.<br/> In humans, IL-10 is encoded by the IL10 gene, which is located on chromosome 1 and comprises 5 exons, and is primarily produced by monocytes and, to a lesser extent, lymphocytes, namely type-II T helper cells (TH2), mast cells, CD4+CD25+Foxp3+ regulatory T cells, and in a certain subset of activated T cells and B cells.<br/> IL-10 is a cytokine with multiple, pleiotropic, effects in immunoregulation and inflammation. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. |
| Uniport ID | P22301 |
| Research Area | Immunology |
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