Human Immunoglobulin G (IgG) Turbidimetric Assay Kit (E-BC-K812-M)
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For research use only.
| Detection Principle |
The quantification of immunoglobulin G (IgG) in serum is of great significance for the diagnosis of primary or secondary immune deficiencies, the monitoring of immunoglobulin therapy, and the clinical course of multiple myeloma. The detection principle of this kit: the antigen-antibody reaction between human IgG and IgG antibodies, which forms immune complexes and causes turbidity in the reaction system. This turbidity results in an absorption peak at 570 nm, and the concentration is directly proportional to the absorbance. |
| Sample Type | Serum,Plasma |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (560-580 nm, optimum wavelength: 570 nm) |
| Research Area | Metabolic Diseases |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.84 mg/mL |
| Detection Range | 0.84-40.00 mg/mL |
| Precision | inter-assay CV: 7.1%-9.9% | intra-assay CV: 4.6%-5.7% |
| Sample Volume | 2 μL |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Human plasma | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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