Human MT(Melatonin) ELISA Kit (E-EL-H2016)

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human MT. During the reaction, Human MT in the sample or standard competes with a fixed amount of Human MT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human MT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human MT in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Melatonin is a methoxyindole synthesized and secreted principally by the pineal gland at night under normal light无dark conditions.   The endogenous rhythm of secretion is generated by the suprachiasmatic nuclei and entrained to the light无dark cycle.   Light is able to either suppress or synchronize melatonin production according to the light schedule.   The nycthohemeral rhythm of this hormone can be evaluated by repeated measurement of plasma or saliva melatonin or urine sulfatoxymelatonin, the main hepatic metabolite.   The primary physiological function of melatonin, whose secretion adjusts to night length, is to convey information concerning the daily cycle of light and darkness to body structures.   This information is used for the organisation of functions, which respond to changes in the photoperiod such as the seasonal rhythms.   Seasonal rhythmicity of physiological functions in humans related to possible alteration of the melatonin message remains, however, of limited evidence in temperate areas under field conditions.   Also, the daily melatonin secretion, which is a very robust biochemical signal of night, can be used for the organisation of circadian rhythms.   Although functions of this hormone in humans are mainly based on correlations between clinical observations and melatonin secretion, there is some evidence that melatonin stabilises and strengthens coupling of circadian rhythms, especially of core temperature and sleep-wake rhythms.   The circadian organisation of other physiological functions depend also on the melatonin signal, for instance immune, antioxidant defences, haemostasis and glucose regulation.   The difference between physiological and pharmacological effects of melatonin is not always clear but is based upon consideration of dose and not of duration of the hormone message.   It is admitted that a "physiological" dose provides plasma melatonin levels in the same order of magnitude as a nocturnal peak.   Since the regulating system of melatonin secretion is complex, following central and autonomic pathways, there are many pathophysiological situations where melatonin secretion can be disturbed.   The resulting alteration could increase the predisposition to disease, add to the severity of symptoms or modify the course and outcome of the disorder.