Human NFE2L2(Nuclear Factor, Erythroid Derived 2, Like 2) CLIA Kit (E-CL-H1005)

Name: Human NFE2L2(Nuclear Factor, Erythroid Derived 2, Like 2) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1005
Price: 180.0 USD
Availability: InStock

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Human NFE2L2(Nuclear Factor, Erythroid Derived 2, Like 2) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human NFE2L2 in samples.No significant cross-reactivity or interference between Human NFE2L2 and analogues was observed
Recovery	80%-120%
Sample Type	Tissue homogenates, cell lysates and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	53257.000	62691.000	57974.000	57937
1000	24372.000	25842.000	25107.000	25070
500	12419.000	10413.000	11416.000	11379
250	5172.000	6006.000	5589.000	5552
125	2792.000	2534.000	2663.000	2626
62.5	1446.000	1412.000	1429.000	1392
31.25	689.000	721.000	705.000	668
0	37.000	37.000	37.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	109.030	295.140	683.640	100.620	322.590	743.070
Standard deviation	11.370	33.290	63.990	10.900	28.360	66.650
CV（%）	10.430	11.280	9.360	10.830	8.790	8.970

Recovery

The recovery of Human NFE2L2 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	98-111	105
EDTA plasma （n=8）	100-114	107
Cell culture media （n=8）	86-98	93

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human NFE2L2 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	93-106	94-106	102-117
1:2	Average	100	100	108
1:4	Range	100-118	102-119	103-115
1:4	Average	108	108	109
1:8	Range	100-114	95-110	93-105
1:8	Average	106	101	99

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NFE2L2. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NFE2L2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NFE2L2, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human NFE2L2. You can calculate the concentration of Human NFE2L2 in the samples by comparing the RLU value of the samples to the standard curve.

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