Human PAF(Platelet Activating Factor) ELISA Kit (E-EL-H2199)

Name: Human PAF(Platelet Activating Factor) ELISA Kit
Brand: Elabscience
SKU: E-EL-H2199
Price: 150.0 USD
Availability: InStock

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Human PAF(Platelet Activating Factor) ELISA Kit - 1

Size	Price	Qty
96T	$ 609.00	- +
48T	$ 487.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	46.88 pg/mL
Detection Range	78.13-5000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human PAF in samples. No significant cross-reactivity or interference between Human PAF and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
5000	2.361	2.291	2.326	2.246
2500	1.662	1.666	1.664	1.584
1250	0.890	0.854	0.872	0.792
625	0.573	0.551	0.562	0.482
312.5	0.292	0.298	0.295	0.215
156.25	0.184	0.182	0.183	0.103
78.13	0.132	0.132	0.132	0.052
0	0.078	0.082	0.080	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	227.480	491.830	2372.540	217.930	527.970	2533.400
Standard deviation	14.900	33.790	106.290	13.730	45.770	168.470
CV（%）	6.550	6.870	4.480	6.300	8.670	6.650

Recovery

The recovery of Human PAF was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Cell culture media （n=8）	92-104	97
Serum （n=8）	85-98	92
EDTA plasma （n=8）	94-109	101

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human PAF and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	95-108	95-108	97-113
1:2	Average	103	103	104
1:4	Range	89-102	80-91	98-112
1:4	Average	97	86	105
1:8	Range	96-109	85-96	98-116
1:8	Average	102	90	106

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	91.67-114.41
Sample 2（n=16）	89.82-112.79

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PAF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PAF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PAF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PAF. You can calculate the concentration of Human PAF in the samples by comparing the OD of the samples to the standard curve.

Background

Platelet-activating factor (PAF) is a phospholipid mediator and has a wide spectrum of biological properties. PAF is produced by a variety of cells including platelets, endothelial cells, and macrophages. In the vascular system, it increases the permeability of monolayer endothelial cells, activates monocytes无 macrophages and polymorphonuclear neutrophils (PMN), and stimulates the contraction of smooth muscle cells .