Human PD-L2 (Programmed Cell Death Protein 1 Ligand 2) ELISA Kit

Size	Price	Qty
96T	$ 495.00
48T	$ 396.00
24T	$ 150.00
96T*5	Inquire	/
96T*10	Inquire	/

Product Summary

Sensitivity	0.09 ng/mL
Detection Range	0.16-10 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reacitivity	Human
Specificity	This kit recognizes PD-L2 in samples.No significant cross-reactivity or interference between PD-L2 and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PD-L2 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PD-L2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PD-L2 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of PD-L2 . You can calculate the concentration of PD-L2 in the samples by comparing the OD of the samples to the standard curve.

Background

Programmed cell death 1 ligand 2(also known as PD-L2, B7-DC) is aproteinthat in humans is encoded by thePDCD1LG2gene.PDCD1LG2 has also been designated asCD273(cluster of differentiation273). Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.

Uniport ID	Q9BQ51
Research Area	Immunology

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Human PD-L2 (Programmed Cell Death Protein 1 Ligand 2) ELISA Kit (E-EL-H6283)

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