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Human SCCA1(Squamous Cell Carcinoma Antigen 1) ELISA Kit (E-EL-H1721)

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All Size Price Qty
96T $ 609.00
48T $ 487.00
24T $ 150.00
96T*5 Inquire /
96T*10 Inquire /
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For research use only.

Product Summary
Sensitivity 46.88 pg/mL
Detection Range 78.13-5000 pg/mL
Sample Volume 100 μL
Total Assay Time 3 h 30 min
Reacitivity Human
Specificity This kit recognizes Human SCCA1 in samples. No significant cross-reactivity or interference between Human SCCA1 and analogues was observed
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Sandwich
Assay Type Sandwich-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃
Expiration Date 12 months
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SCCA1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SCCA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SCCA1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SCCA1. You can calculate the concentration of Human SCCA1 in the samples by comparing the OD of the samples to the standard curve.
SCC associated antigen generally refers to Squamous cell carcinoma and is a tumor marker. SCC is a glycoprotein that is present in squamous cell carcinoma cells and esophageal cancer cells as an acidic glycoprotein and a tumor marker of squamous cell carcinoma. The normal value is between 0 and 1.5ug/L, if there is an increase in the phenomenon, it may be caused by malignant tumors, such as cervical cancer, lung cancer, skin cancer, etc.
Uniport ID P29508
Research Area Cancer , Cell Biology
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