Hydrogen Peroxide (H2O2) Fluorometric Assay Kit (E-BC-F001)

For research use only.
Detection Principle | In the presence of peroxidase, hydrogen peroxide reacts with the fluorescent probe, and the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 587 nm is proportional to the hydrogen peroxide concentration. |
Sample Type | Serum,Plasma,Tissue,Cell |
Detection Method | Fluorometric method |
Detection Instrument | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
Research Area | Oxidative Stress , Plant Physiology Series , Ferroptosis |
Storage | This product can be stored at 2-8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
Valid Period | 12 months |
Sensitivity | 0.02 μmol/L |
Detection Range | 0.02-10 μmol/L |
Precision | inter-assay CV: 3.6% | intra-assay CV: 1.1% |
Assay Time | 50 min |
The recommended dilution factor for different samples is as follows (for reference only):
Sample Type | Dilution Factor |
---|---|
Human serum | 1-3 |
Mouse serum | 1-4 |
Mouse plasma | 1-2 |
Porcine serum | 1-3 |
Rat serum | 1-4 |
HepG2 culture supernatant | 1 |
10% Mouse liver tissue homogenate | 1-3 |
10% Mouse brain tissue homogenate | 1-3 |
10% Rat lung tissue homogenate | 1-3 |
HepG2 cells homogenate (the concentration of protein is 6.44 gprot/L) | 1-2 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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