Immunol Fluorescence Staining Kit (Anti-Rabbit IgG-Cyanine3) (E-IR-R321)
For research use only.
Product Introduction |
Immuno Fluorence Staining Kits are developed for immunofluorescence detection of cell or tissue sections. When there is an appropriate antibody to detect specific target protein, fluorescence can be detected by immunofluorescence staining kit.
Immuno Fluorescence Staining Kit (Anti-Rabbit IgG-Cyanine3) contains Goat Anti-Rabbit IgG(H+L) (Cyanine3 conjugated), this secondary antibody can detect primary antibody from rabbit with bright red fluorescence. Cyanine3 is a newly red fluorescent probe. It is brighter than most of other red fluorescent probes, more difficult to quench, and has a lower background. The kit contains anti fluorescence quenching sealing solution, which can make the fluorescence more lasting. |
Applications | IF |
Storage | Store at 2~8/-20°C, Refer to the label. Valid for 12 months. Secondary antibody (E-AB-1010) and DAPI Reagent (1 μg/mL) (E-IR-R103) should be stored at -20°C and avoid light. |
Shipping | Biological ice pack at 4 ℃ |
Cautions |
1. This result should be observed by fluorescence microscope. 2. Anti-Fluorescence Quenching Reagent can slow down the quenching, but avoid light and especially shorten the result observation time is still needed. 3. If it can't be observed in time, please store the slice at 4°C and avoid light and observe in one week. 4. If the fluorescence is too weak, increase the primary antibody concentration properly. If the fluorescence is still weak, increase the secondary antibody concentration appropriately. 5. The reagents for immunofluorescence staining and the cover glass and slides should be prepared in advance. 6. For your safety and health, please wear the lab coat and disposable gloves before the experiments. |
Other Clones
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Other Formats
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Biotin
Chromogenic Biotin
Cyanine 3
Cyanine 5
Cyanine 5.5
Cyanine 7
Elab Fluor®405
Elab Fluor®430
Elab Fluor®488
Elab Fluor®568
Elab Fluor®594
Elab Fluor®647
Elab Fluor®680
Elab Fluor®700
Elab Fluor®750
Elab Fluor®Red 780
Elab Fluor®Violet 450
FITC
HRP
NHS-Biotin
NHS-LC-LC-Biotin
Sulfo-NHS-Biotin
Sulfo-NHS-LC-LC-Biotin
Unconjugated
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