IP/CoIP Kit (Agarose) (EA-IP-K007)
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For research use only.
| Applications | IP, COIP |
| Storage | -20℃, 12 months |
| Shipping | ice bag |
| Cautions |
1. Transportation and preservation: This reagent kit is transported under refrigerated conditions. After receiving the product, please remove the purification column C and store it at room temperature; Store gels at -20°C and other components at of the kit 4°C. 2. Suggestions for the use of reagents: P10 (PBS Buffer, pH7.4 (10×)) and P10T (PBST Buffer, pH7.4 (10×)) need to be diluted into 1 × working solution with deionized water before use. 3. Recommendations for the use of Protein A/G gel: Do not freeze or dry the gel, do not sonicate the gel, and do not treat the gel with acid for more than 10min. 4. Acidic eluent selection: It has been shown that Arginine-HCL at pH 3.0 can reduce protein denaturation and prolong the lifetime of affinity gels compared to traditional Glycine-HCL eluents. You can also choose your own acidic eluent according to the actual situation. 5. Affinity of Protein A/G binding to IgG of various species: The binding affinity of antibodies (IgG, IgM, IgA, IgD) to Protein A/G varies among different species. Please carefully read the attachment of this manual before use. |
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