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IRX2 Polyclonal Antibody (E-AB-52041)

All Size Price Qty
200μL $ 399.00
120μL $ 240.00
60μL $ 143.00
20μL $ 73.00
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For research use only.

Verified Samples Verified Samples in WB: Mouse lung
Verified Samples in IHC: Human liver cancer, Human brain
Dilution WB 1:500-1:2000,  IHC 1:30-1:150
Clonality Polyclonal
Immunogen Synthetic peptide of human IRX2
Abbre IRX2
Synonyms Homeodomain protein IRXA2,  IRX 2,  IRX2,  Iroquois homeobox 2,  Iroquois homeobox protein 2,  Iroquois-class homeodomain protein irx-2,  IrxA2,  irx2
Swissprot
Calculated MW 49 kDa
Observed MW Refer to figures
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Nucleus.
Concentration 1.26 mg/mL
Buffer PBS with 0.05% NaN3 and 40% Glycerol,pH7.4
Purification Method Antigen affinity purification
Research Areas Epigenetics and Nuclear Signaling
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background IRX2 is a member of the Iroquois homeobox gene family. Members of this family appear to play multiple roles during pattern formation of vertebrate embryos.IRX2 (Iroquois Homeobox 2) is a Protein Coding gene. Diseases associated with IRX2 include Kidney Clear Cell Sarcoma. GO annotations related to this gene include sequence-specific DNA binding. An important paralog of this gene is IRX5.
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Unconjugated

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