α-Ketoglutarate Dehydrogenase (α-KGDH) Activity Colorimetric Assay Kit (E-BC-K912-M)
For research use only.
| Detection Principle | α-Ketoglutarate Dehydrogenase (α-KGDH) is a key enzyme in the tricarboxylic acid (TCA) cycle. α-KGDH catalyzes the conversion of α-ketoglutarate and NAD+to NADH and succinyl-CoA. Concurrently, NADH transfers hydrogen to convert WST-8 into the chromogenic formazan, and the activity of α-KGDH is determined by measuring the absorbance at 450 nm. |
| Sample Type | Serum,Plasma,Animal tissue,Plant tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (440-460 nm) |
| Research Area | Energy Metabolism , Metabolic Enzyme Activity , Carbohydrate Metabolism |
| Other Reagents Required | Double distilled water |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.054 U/L |
| Detection Range | 0.054-40 U/L |
| Precision | inter-assay CV: 5.2%-7.3% | intra-assay CV: 3.1%-4.5% |
| Sample Volume | 20 μL |
| Assay Time | 1 h 10 min |
Note: Data for this module come from published literature that cites this product, displaying other products used together in the same research paper.
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenization | 1 |
| 10% Mouse kidney tissue homogenization | 1 |
| 10% Mouse heart tissue homogenization | 1 |
| 10% Mouse lung tissue homogenization | 1 |
| 1×10^6 RAW264.7 cells | 1 |
| 1×10^6 Jurkat cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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