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For research use only.

Verified Samples Verified Samples in IHC: Rat ovary, Rat heart, Human colon carcinoma, Human appendix, Human breast cancer, Human stomach
Verified Samples in IF: L929, U-2OS
Dilution IHC 1:50-1:200,  IF 1:50-1:200
Isotype IgG
Host Rabbit
Reactivity Human,  Mouse,  Rat
Applications IHC,  IF
Clonality Polyclonal
Immunogen Recombinant fusion protein of human KIF14 (NP_055690.1).
Abbre KIF14
Synonyms KIF14,  MKS12
Swissprot
Cellular Localization Nucleus, Cytoplasm, Cytoplasm, cytoskeleton, spindle, Nuclear localization observed during interphase in PubMed: 16431929 or triggered by entry into mitosis in PubMed: 16648480, Cytoplasmic in interphase (PubMed: 16648480) and metaphase cells (PubMed: 16431929), From prophase to metaphase, accumulates at the developing spindle poles and their associated microtubules, During anaphase, accumulates at the spindle midzone, Localization to the central spindle and midbody during anaphase is dependent upon PRC1 and CIT presence, In cells ready to undergo abscission, concentrates at the contractile ring.
Concentration 1 mg/mL
Buffer Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol.
Purification Method Affinity purification
Research Areas Cancer,  Signal Transduction,  Tags and Cell Markers
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background This gene encodes a member of the kinesin-3 superfamily of microtubule motor proteins. These proteins are involved in numerous processes including vesicle transport, chromosome segregation, mitotic spindle formation, and cytokinesis. In human HeLa-S3 and 293T cells, this protein is localized to the cytoplasm during interphase, to the spindle poles and spindle microtubules during mitosis, and to the midbody during cytokinesis. An internal motor domain displays microtubule-dependent ATPase activity, consistent with its function as a microtubule motor protein. Knockdown of this gene results in failed cytokinesis with endoreplication, which results in multinucleated cells. This gene has been identified as a likely oncogene in breast, lung and ovarian cancers, as well as retinoblastomas and gliomas. Alternative splicing results in multiple transcript variants.
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Unconjugated

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