KRR1 Polyclonal Antibody (E-AB-65481)
For research use only.
| Verified Samples |
Verified Samples in WB: HeLa, 293T, A431, HepG2, Mouse thymus Verified Samples in IF: U2OS |
| Dilution | WB 1:500-1:2000, IF 1:50-1:200 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IF |
| Clonality | Polyclonal |
| Immunogen | Recombinant fusion protein of human KRR1 (NP_008974.5). |
| Abbre | KRR1 |
| Synonyms | HRB2, KRR1, RIP-1 |
| Swissprot | |
| Calculated MW | 36 kDa/43 kDa |
| Observed MW |
44 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus, nucleolus. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Epigenetics and Nuclear Signaling, Immunology, Tags and Cell markers |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | The SSU is a large ribonucleoprotein consisting of at least 40 proteins and the U3 small nucleolar RNA. It is involved in pre-rRNA processing and ribosome assembly. The SSU is necessary for the biogenesis of the 18S rRNA. Cells that are depleted of SSU proteins will arrest in the G1 phase of the cell cycle. KRR1, also known as HRB2 (HIV-1 Rev binding protein 2) or RIP-1 (Rev interacting protein 1), is a nonribosomal component of the small subunit processome (SSU). KRR1 is 381 amino acids in length and is evolutionarily conserved among human, yeast, fly, nematode and rice. KRR1 localizes to the nucleolus and is highly expressed in dividing cells. It contains one conserved KH domain (RNA-binding motif) and is a crucial component of the SSU, required for both rRNA maturation and ribosome biogenesis. |
Other Clones
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Other Formats
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Unconjugated
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