L-Arginine (L-Arg) Colorimetric Assay Kit (E-BC-K850-M)
For research use only.
| Detection Principle | Arginine (Arg) is an essential amino acid in the human body and can also be absorbed in food. Natural arginine is L-type, which is alkaline and easily reacts with acids to form salts. Arginine is an intermediate metabolite in the ornithine cycle, which can promote the conversion of ammonia into urea, thereby reducing blood ammonia content.Arginine generates urea under the action of enzymes, and urea can complex with oxime reagents under strong acidic conditions by boiling. The complex has a maximum absorption peak at 520 nm, and the arginine content can be calculated by measuring the OD value at 450 nm. |
| Synonyms | L-Arg |
| Sample Type | Serum,Plasma,Urine,Plant,Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (510-530 nm, optimum wavelength: 520 nm) |
| Research Area | Amino Acids And Proteins |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.008 mmol/L |
| Detection Range | 0.008-1 mmol/L |
| Precision | inter-assay CV: 6-8% | intra-assay CV: 1.8-3.8% |
| Sample Volume | 80 μL |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Rat liver tissue homogenate | 1-2 |
| 10% Rat kidney tissue homogenate | 1-2 |
| 10% Rat heart tissue homogenate | 1-2 |
| 10% Rat lung tissue homogenate | 1-2 |
| Human serum | 1-2 |
| Human plasma | 1-2 |
| Bovine serum | 1-2 |
| 10% Nartjie tissue homogenate | 1-2 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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