Lactase Activity Colorimetric Assay Kit (E-BC-K865-S)
For research use only.
| Detection Principle | Lactase decomposes lactose to produce glucose. Under the action of enzyme, glucose produces hydrogen peroxide. In the presence of chromogenic oxygen receptors, peroxidase catalyzes hydrogen peroxide to produce colored substances. There is a characteristic absorption peak at 505 nm, and the magnitude of enzyme activity is directly proportional to the OD value. |
| Sample Type | Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (505 nm) |
| Research Area | Glycolysis And Lipid Metabolism |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.10 U/L |
| Detection Range | 0.10-125 U/L |
| Precision | inter-assay CV: 5.0%-7.0% | intra-assay CV: 3.6%-4.8% |
| Sample Volume | 50 μL(tissue homogenate) |
| Assay Time | 60 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse small intestinal tissue homogenate | 1-2 |
| 10% Mouse large intestinal tissue homogenate | 1-2 |
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Mouse spleen tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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